蛇毒液中透明质酸酶的电喷雾质谱测定

@汪聪慧 $公安部物证鉴定中心!北京100038@Supason Pattannargson$ Dept.of Chemistry Faculty of Science!Chulalongkorn Univ., Bangkok,Tailand@马龙华 $Div.of Neoplastic Disease,Dept.of Medicine,Mount Sinai School of Medicine!New York 10029 USA@John Roboz$ Div.of Neoplastic Disease,Dept.of Medicine,Mount Sinai School of Medicine!NEW YORK 10029 USA

ELECTROSPRAY MS OF HYALURONIDASE IN SNAKE VENOMS

Abstract: Hyaluronidases (hyalases) from bovine testes and leeches were used as "standard". Constituents of crude snake venoms-hyalases were separated by size exclusion HPLC. Molecular masses of hyalases from snake venoms, determined by on-line capillary HPLC-mass spectrometry with electrospray ionization, were: Naja melanoleuca (black cobra) 49377Da, Naja Kaothia(Thai cobra)60265Da, Sepedon hemachatus (ringhals cobra) 49722Da. Mass determinations were repeatable within 0.03%.