Abstract: Different from mass spectrometry, whose separation is depended on the mass to charge, the ion mobility spectrometry(IMS) separate gaseous ions based on their size and shape. Thus the combination of IMS and MS can provide more information of protein structure. The travelling wave ion mobility spectrometry(TWIMS) is a new member of IMS family with good sensitivity and quick analysis rate. TWIMS-MS is suitable for analyzing the large molecular structure at biological concentration and within biological timescales, and its applications of protein complex structure and protein folding progress research have been paid great attention.