尼泊尔黄堇中总生物碱的串联质谱分析

Tandem Mass Spectrometry Analysis of Total Alkaloids from Corydalis hendersonii

摘要: 采用电喷雾串联质谱（ESI-MS/MS）法对尼泊尔黄堇中总生物碱进行分析。由一级质谱中各成分的准分子离子峰获知其相对分子质量，再由二级质谱的裂解碎片推测其结构特征。结合纯品的串联质谱分析，直接鉴定出从尼泊尔黄堇总生物碱中已经分离得到的10种异喹啉生物碱成分：stylopine(1)，tetrahydrothalifendine(2)，tetrahydroberberrubine(3)，scoulerine(4)，sinactine(5)，protopine(6)，cryptopine(7)，α-allocryptopine(8)，9-methyl-decumbenine C(9)和6-acetonyl-5,6-dihydrosanguinarine(10)；另外还初步推测出2种未从该植物中分离得到的异喹啉生物碱成分。

Abstract: Electrospray ionization tandem mass spectrometry(ESI-MS/MS) was used to analyze the total alkaloids from Corydalis hendersonii. Based on the information of pseudo molecular ions and daughter ions, which were obtained from ESI-MS and tandem mass spectrometry， total of 10 main alkaloids are suggested as stylopine(1), tetrahydrothalifendine(2), tetrahydroberberrubine(3), scoulerine(4), sinactine(5), protopine(6), cryptopine(7), α-allocryptopine(8), 9-methyl-decumbenine C(9), and 6-acetonyl-5,6-dihydrosanguinarine(10), respectively.