UPLC-MS/MS同位素内标法测定食品中对位红、苏丹红Ⅰ~Ⅳ的研究

UPLC-MS/MS Isotopic Internal Standard Determination of Parared and SudanⅠ-Ⅳ in Food

摘要: 建立了食品中对位红、苏丹红I～Ⅳ的超高效液相色谱电喷雾串联质谱测定方法，并采用氘代苏丹红作为同位素内标进行定量。样品经提取、液液萃取和固相萃取（Waters OasisMAX）净化，用超高效液相色谱分离后，采用电喷雾串联质谱进行定性和定量检测。线性范围为0～100 μg•L^-1，线性相关系数r大于0.99，方法的定性检出限均小于1 μg•kg^-1(S/N=10)，高、中、低3个浓度水平的加标回收率为78%～112.4％。

Abstract: A sensitive method for determination of Parared and SudanⅠ-Ⅳ in food was proposed by UPLC-ESI-MS/MS with isotopic internal standard of sudanred. The food sample was extracted and purified by liquid phase extraction and solid-phase extraction (Waters OasisMAX), then was determined by UPLC-ESI-MS/MS. The calibration curves of Parared and SudanⅠ-Ⅳ show good linearity in the range of 0.1-100 μg•L^-1 with correlation of more than 0.99. The detection limit of method is less than 1 μg•kg^-1(S/N=10). The recoveries in three levels range from 78% to 112.4%.