Abstract: In recent years, there is increased attention for accurate measurement of total amount, isotopic composition and speciation of selenium in various samples such as biological, environmental, geological, food samples and so on. With the development of mass spectrometry techniques, inductively coupled plasma mass spectrometry (ICP-MS) becomes a powerful tool for research and measurement of property values of Se, and may be enabling new insights and broad application in life sciences, environmental geochemistry, biology, food for Se studies. This review presents the latest advance of ICP-MS in determination of total amount, isotopic composition and speciation of Se.