Changes of Chemical Constituents in <i>Scrophulariae Radix </i>During Processing Based on UPLC-Q-TOF MS

WANG Jing-zhe; LIU Zhen; MA Li-man; LI Jun; SHANG Ming-ying; LIU Guang-xue; XU Feng; CAI Shao-qing

doi:10.7538/zpxb.youxian.2015.0042

Journal of Chinese Mass Spectrometry Society > 2016 > 37(1): 1-9. > DOI: 10.7538/zpxb.youxian.2015.0042 CSTR: 32365.14.zpxb.youxian.2015.0042

WANG Jing-zhe, LIU Zhen, MA Li-man, LI Jun, SHANG Ming-ying, LIU Guang-xue, XU Feng, CAI Shao-qing. Changes of Chemical Constituents in Scrophulariae Radix During Processing Based on UPLC-Q-TOF MS[J]. Journal of Chinese Mass Spectrometry Society, 2016, 37(1): 1-9. DOI: 10.7538/zpxb.youxian.2015.0042

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Changes of Chemical Constituents in Scrophulariae Radix During Processing Based on UPLC-Q-TOF MS

School of Pharmaceutical Sciences, Peking University, Beijing 100191, China

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Abstract

In order to clarify the influence of the traditional processing on the chemical constitution of Scrophulariae Radix, the UPLC-Q-TOF MS method was used to analyze the dried and processed Scrophulariae Radix. Data were processed by principal component analysis (PCA) and orthogonal partial least squared discriminant analysis (OPLS-DA) to find the difference between dried and processed Scrophulariae Radix. The accurate m/z values of Q-TOF MS and Q-TOF MS/MS fragments were applied to constitution identification. The results show that a total of 26 constituents have significant differences between dried and processed Scrophulariae Radix. Fifteen constituents are identified, including 9 iridoid glycosidesand 6 phenylpropanoid glycosides. Compared with the dried Scrophulariae Radix, the contents of aucubin, 6-O-methylcatapol, harpagoside, 8-O-cumaroylharpagide, 8-O-feruloylharpagide, acteoside, isoacteoside, angoroside C and scrophuloside B₁ in processed Scrophulariae Radixwere are decreasing. The contents of harpagide, harp agoside isomer, 6″-O-α-D-galactopyranosylharp agoside, 6-O-α-D-galactopyranosylharp agoside, sibirioside A and cinnamic acid are increasing. In Scrophulariae Radix, scrophuloside B₁ is detected for the first time. The UPLC/Q-TOF-MS method proves to have a high sensitivity and the result isaccurate and reliable. The method could fully reflect the changes of multiple chemical constituents in the course of processing and lay a good foundation for the further study of processing mechanism of traditional Chinese medicine.
- Scrophulariae Radix,
- processing,
- UPLC-Q-TOF MS,
- PCA,
- OPLS-DA

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[1]	肖培根. 新编中药志[M]. 北京:化学工业出版社,2002:366.
[2]	国家药典委员会. 中国药典[M]. 2010版. 北京:中国医药科技出版社,2010:108-109.
[3]	姜守刚,蒋建勤,祖元刚. 玄参的化学成分研究[J]. 植物研究,2008,28(2):254-256.JIANG Shougang, JIANG Jianqin, ZU Yuangang. Chemical components from the root of Scrophularianingpoensis[J]. Bulletin of Botanical Research, 2008, 28(2): 254-256(in Chinese)．
[4]	李医明,蒋山好,高文远,等. 玄参中的苯丙素苷类成分[J]. 中草药,1999,30(7):487-489.LI Yiming, JIANG Shanhao, GAO Wenyuan, et al. Phenylpropanoid glycosides of ningpo figwort (Scrophularianingponesis)[J]. Chinese Traditional and Herbal Drugs, 1999, 30(7): 487-489(in Chinese).
[5]	胡瑛瑛,黄真. 玄参的化学成分及药理作用研究进展[J]. 浙江中医药大学学报,2008,32(2):268-270.HU Yingying, HUANG Zhen. Advances in research of chemical constituents and pharmacological effects of Scrophularia Ningpoensis Hemsl[J]. Journal of Zhejiang College of Traditional Chinese Medicine, 2008, 32(2): 268-270(in Chinese).
[6]	王建华,谢丽华,钱瑞琴,等. 玄参中哈巴苷和哈巴俄苷滋阴作用的药理研究[C]. 全国中药研究与开发学术研讨会论文集，2001:47.
[7]	李医明,曾华武,贺祥,等. 玄参中环烯醚萜甙和苯丙素甙对LTB4产生及血小板聚集的影响[J]. 第二军医大学学报,1999,20(5):301-303.LI Yiming, ZENG Huawu, HE Xiang, et al. Iridoid and phenylpropanoid glycosides of Scrophularianingpoensis inhibit the formation of LTB4 and platelet aggregation[J]. Academic Journal Second Millitary Medical University, 1999, 20(5): 301-303(in Chinese).
[8]	王珲,陈平,张丽萍,等. 玄参多糖成分抗疲劳活性的研究[J]. 武汉植物学研究,2009,27(1):118-120.WANG Hui, CHEN Ping, ZHANG Liping, et al. Study on the antifatigue function of polysaccharides from Scrophularianingpoensis Hemsl[J]. Journal of Wuhan Botanical Research, 2009, 27(1): 118-120(in Chinese).
[9]	方学敏,曹岗,蔡银燕. 玄参炮制前后多糖含量的变化研究[J]. 中华中医药学刊,2012,30(5):986-987.FANG Xuemin, CAO Gang, CAI Yinyan.Studies on the change of the content of polysaccharides in crude and processed Scrophulariae Radix[J]. Chinese Archivesof Traditional Chinese Medicine, 2012, 30(5): 986-987(in Chinese).
[10]	王建华,谢丽华,刘洪宇,等. 玄参不同加工品中哈巴俄苷与肉桂酸的HPLC含量测定[J]. 中国药学杂志,2000,35(6):375-378.WANG Jianhua, XIE Lihua, LIU Hongyu, et al. HPLC determination of harpagoside and cinnamic acid in Xuanshen after processed in different methods[J]. Chinese Pharmaceutical Journal, 2000, 35(6): 375-378(in Chinese).
[11]	白云娥,袁鹏飞,王庆辉. HPLC-UV波长转换法测定玄参药材及饮片中哈巴苷与哈巴俄苷的含量[J]. 中国中药杂志,2011,36(19):2697-2702.BAI Yun’e, YUAN Pengfei, WANG Qinghui, et al. Determination of harpagide and harpagosidein Scrophulariae Radix by HPLC-UV[J]. China Journal of Chinese Materia Medica, 2011, 36(19): 2697-2702(in Chinese).
[12]	WU Q, YUAN Q, LIU E H, et al. Fragmentation study of iridoid glycosides and phenylpropanoid glycosides in Radix Scrophulariae by rapid resolution liquid chromatography with diode-array detection and electrospray ionization time-of-flight mass spectrometry[J]. Biomedical Chromatography, 2010, 24(8): 808-819.
[13]	TOSHIO M, AI M.Acylatediridoid and phenylethanoidglycosides from the aerial parts of Scrophularia nodosa[J]. Journal of Natural Products, 1999, 62(8): 1079-1084.
[14]	HAN L F, MAVIS B Y, LIU E W, et al. Structural characterisation and identification of phenylethanoidglycosides from Cistanchesdeserticola Y.C. Ma by UHPLC/ESI-QTOF-MS/MS[J]. Phytochemical Analysis, 2012, 23(6): 668-676.
[15]	LI Y M, JIANG S H, GAO W Y, et al. Phenylpropanoid glycosides from Scrophularia ningpoensis[J]. Phytochemistry, 2000, 54(8): 923-925.
[16]	NIU Z R, WANG R F, SHANG M Y, et al. A new iridoid glycoside from Scrophularianingpoensis[J]. Natural Product Research, 2009, 23(13): 1181-1188.
[17]	CHEN B, WANG N L, HUANG J H, et al. Iridoid and phenylpropanoid glycosides from Schrophularianingpoensis Hemsl[J]. Asian Journal of Traditional Medicines, 2007, 2(3): 118-123.
[18]	CHEN B, LIU Y, LIU H W, et al.Iridoid and aromatic glycosides from Scrophularianingpoensis Hemsl and their inhibition of [Ca²⁺] increase induced by KCl[J]. Chemistry & Biodiversity, 2008, 5(9): 1723-1735.
[19]	SO R K, LEE K Y, KOO KA, et al. Four new neuroprotectiveiridoid glycosides from Scrophulariabuergerianaroots[J]. Journal of Natural Products, 2002, 65(11): 1692-1699.
[20]	LI Y M, JIANG S H, GAO W Y, et al. Iridoid glycosides from Scrophularianingpoensis[J].Phytochemistry, 1998, 50(1): 101-104.
[21]	CHEN L, WANG D, WU J, et al. Identification of multiple constituents in the traditional Chinese medicine formula GuiZhiFuLing-Wan by HPLC DAD MS/MS[J]. Journal of Pharmaceutical and Biomedical Analysis, 2009, 49(2): 267-275.
[22]	CAO G, CONG X D,CAI H, et al. Simultaneous quantitation of eight active components in crude and processed Radix Scrophulariae extracts by high performanceliquid chromatography with diode array detector[J]. Chinese Journal of Natural Medicines, 2012, 10(3): 213-217.

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