A Data Processing Tool for Mass Spectrometry Data from iSRM Strategy in Proteomics

In proteomics research, selected reaction monitoring (SRM) technology based on mass spectrometry has been widely applied in the protein quantitative analysis because of its high reproducibility and accuracy. Intelligent selected reaction monitoring (iSRM) technology is a new SRM experimental strategy. In order to process the mass spectrometry data produced by iSRM, a peptide quantitative information extraction tool called iSQuant is developed. iSQuant is written with MATLAB programming language, and thus is convenient and user-friendly. Finally, a replicated dataset and a standard dataset are used to evaluate the performances of iSQuant. The results show that iSQuant has superior reproducibility and linearity.