Simultaneous Determination of the Estrogen, Progestogen and Androgen in Aquatic Products by Isotope Dilution LC-MS/MS

YU Hui-juan; ZHANG Shan-shan; ZHANG Xiao-ling; HUI Yun-hua; GU Run-run; FENG Bing

doi:10.7538/zpxb.2014.35.02.0170

Journal of Chinese Mass Spectrometry Society > 2014 > 35(2): 170-178. > DOI: 10.7538/zpxb.2014.35.02.0170 CSTR: 32365.14.zpxb.2014.35.02.0170

YU Hui-juan, ZHANG Shan-shan, ZHANG Xiao-ling, HUI Yun-hua, GU Run-run, FENG Bing. Simultaneous Determination of the Estrogen, Progestogen and Androgen in Aquatic Products by Isotope Dilution LC-MS/MS[J]. Journal of Chinese Mass Spectrometry Society, 2014, 35(2): 170-178. DOI: 10.7538/zpxb.2014.35.02.0170

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Simultaneous Determination of the Estrogen, Progestogen and Androgen in Aquatic Products by Isotope Dilution LC-MS/MS

1.
East China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences, Shanghai 200090, China
2.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China

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Abstract

A method of liquid chromatography-tandem mass spectrometry（LC-MS/MS） was developed for the simultaneous determination of estrogen, progestogen and androgen in aquatic products muscle tissues. The sample were extracted by tert-butyl methyl ether and ethyl acetate, purified on C18 solid phase extraction column, eluted by methanol. After XBridge RP-18 column separation using 0.02% ammonia-methanol as mobile phase, the eluents were qualitatively and quantitatively determined under multi-reaction monitoring scan type with tandem mass analyzer using positive polarity mode for the progestogen, androgen and estradiol benzoate, negative polarity for the estrogen respectively, the quantification was performed with internal standard method. The limit of detection is 0.5—2 μg/kg（S/N≥3）. Spike recoveries are 73.0%—120% and 79.0%—108% in 2 μg/kg and 10 μg/kg with relative standard deviation of 5.3%—15% and 2.3%—8.2%. The method is effective, simple and sensitive, and suitable for the determination and confirmation of 27 kinds of sex hormones in aquatic products.
- estrogen,
- progestogen,
- androgen,
- liquid chromatography-tandem mass spectrometry（LC-MS/MS）,
- aquatic products

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Simultaneous Determination of the Estrogen, Progestogen and Androgen in Aquatic Products by Isotope Dilution LC-MS/MS

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