Determination and Application of the Carbon-13 Content of Sugar and Pulp from Orange Juice

Stable carbon isotope analysis is an effective method for identifying the botanic origin of plant product. In this paper, δ¹³C of sugar and pulp of orange juice were determined by elementar analyzer-isotope ratio mass spectrometer(EA-IRMS)， combined with separation technology. The isotope difference between natural and laboratory imitated orange juice was presented. Results show that isotope fractionation is not observed， while sample preparation and the standard deviation are merely 0.1‰. The δ¹³C of pulp is identical to that sugar in the same juice and different with cane sugar significantly, which can be used in the detection of C4 sugar in orange juice.