Determination of 29 Chemical Constituents in the QingGanSanJie Decoction by Accelerated Solvent Extraction and HPLC-ESI-TOF MS

WANG Hui; LI Yun-qing; LI Yang; QIAN Xian; CHAI Yi-feng; ZHANG Guo-qing; LV Lei; ZHAO Liang

doi:10.7538/zpxb.2016.0181

Journal of Chinese Mass Spectrometry Society > 2017 > 38(4): 503-514. > DOI: 10.7538/zpxb.2016.0181 CSTR: 32365.14.zpxb.2016.0181

WANG Hui, LI Yun-qing, LI Yang, QIAN Xian, CHAI Yi-feng, ZHANG Guo-qing, LV Lei, ZHAO Liang. Determination of 29 Chemical Constituents in the QingGanSanJie Decoction by Accelerated Solvent Extraction and HPLC-ESI-TOF MS[J]. Journal of Chinese Mass Spectrometry Society, 2017, 38(4): 503-514. DOI: 10.7538/zpxb.2016.0181

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Determination of 29 Chemical Constituents in the QingGanSanJie Decoction by Accelerated Solvent Extraction and HPLC-ESI-TOF MS

1.
Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China
2.
Department of Pharmacy,The Second Hospital of Shanxi Medical University, Taiyuan 030000, China
3.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China

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Abstract

QingGanSanJie decoction (QGSJ), which consists of 14 different herbs, is a multiherbal formula for liver cancer that is administered in different combinations based on experimental observations and clinical experiences from long-term applications. A method based on accelerated solvent extraction (ASE) followed by high-performance liquid chromatography (HPLC) coupled with electrospray time-of-flight mass spectrometry (ESI-TOF MS) were established for simultaneous determination of 29 chemical components in QGSJ. The operational parameters of ASE, including the extraction solvent (70% ethanol), extraction temperature (100 ℃), static extraction time (5 min), and extraction cycles (two cycles) were optimized by orthogonal design and principal component analysis. HPLC separation was performed on a Kromasil C18 HPLC column with a linear gradient mobile phase system. TOF MS was set in negative ion mode with a detector range of m/z100-1100. The results show that 29 compounds in the QGSJ have good linearity (r>0.994), and intra-day and inter-day precision (relative standard deviation) are less than 5%. The extraction recoveries of the 29 compounds range from 96.5% to 104.5%. The method is rapid and reliable, which is suitable for the quantitative evaluation of multiherbal traditional Chinese medicine.

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Determination of 29 Chemical Constituents in the QingGanSanJie Decoction by Accelerated Solvent Extraction and HPLC-ESI-TOF MS

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