Simultaneous Determination of Nineteen Fatty Acids in Antrodia camphorata Powder by Derivatized GC/MS

A method of derivatized gas chromatography-mass spectrometry (GC/MS) was developed for the simultaneous determinations of nineteen fatty acids in Antrodia camphorata of different batches and different cultivation. The analysis was carried on a HP-VOC capillary column (60 m×320 μm×1.8 μm) with single ion monitoring (SIM) mode. Nineteen fatty acids show good linearity over the tested ranges (r＞0.995 6), and the recovery ranges from 93.47% to 104.89%. This method with good repeatability is found to be selective, accurate and suitable for the determination of fatty acids in Antrodia camphorata powder, which can provide the valuable materials for quality assessment of Antrodia camphorata powder.