Study on Ginsenosides in White Ginseng and Dali Ginseng by RRLC-Q-TOF MS/MS

Rapid resolution liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry (RRLC-Q-TOF MS/MS) was used to compare the white ginseng with dali ginseng in the composition of ginsenosides. Based on the fragmentation law of the isomeric compounds, ginsenoside Rd and Re, ginsenoside Rf and Rg₁, and the feature of ginsenoside mRd in MS/MS, 35 kinds of ginsenosides were identified by the comparison of the retention time of the standard compound and the accurate mass obtained from RRLC-Q-TOF MS/MS. The rare ginsenosides were detected by the comparison of white ginseng with dali ginseng, such as ginsenoside Rg₅， Rk₁, F₄， Rg₆ etc. The methods has reference significance for the research of compounds in the processed products of herbs, like dali ginseng. The results provide the reference for clinical rational drug use.