Simultaneous Determination of Six Kinds of Amadori Compounds in Tobaccos by LC-MS/MS

A liquid chromatography-tandem mass spectrometry(LC-MS/MS) method for qualitatively and quantitatively analyzing six kinds of Amadori compounds (Fru-Gly, Fru-Ala, Fru-Pro，Fru-Thr，Fru-Glu and Fru-Amb) in tobaccos was developed. The sample was extracted with 70% methanol water solutionsand assisted by ultrasound, and the extraction conditions which were optimized by orthogonal experiment as followings: the solid liquid ratio was 1∶30, the extraction power was 120 W，and the extraction time was 25 min. The extraction products from tobaccos were cleaned up by hexane and then analyzed by LC-MS/MS under the selected reaction monitoring (SRM) mode. The contents of six kinds of Amadori compounds were determined by external standard curve method. The results show that the limits of detection (LOD) are 20.06-39.86 μg/kg, and limits of quantity (LOQ) are 66.85-132.9 μg/kg. The correlation coefficients are greater than 0.995 0, the average recoveries are 86.56%-88.86%, and the relative standard deviations（RSD） are less than 5%. This method is simplify, sensitive and accurate, which is suitable for qualitative and quantitative analysis of Amadori compounds in tobaccos.