Difference of Chemical Compositions in Pseudostellariae Radix from Different Origins by UPLC-Triple TOF-MS/MS

The difference of chemical compositions in Pseudostellariae Radix from different origins was researched by UPLC-Triple TOF-MS/MS. Through the analysis of the multistage tandem mass spectrometry, the characteristic peaks were extracted with mass spectrometry data peak matching, peak alignment, noise filtering. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were used for data processing. The components were identified according to MS accurate mass and MS/MS spectrometry fragmentation information, combined with the software of database search and literature. The results show that the chemical compositions in Pseudostellariae Radix from different origins are clearly distinguished. Kinds of 21 chemical compositions are indentified. Among of them, there are 10 kinds of common difference of chemical compositions, such as maltotriose, sucrose, thyronine, inosine triphosphate, pseudostellarin A, pseudostellarin B, pseudostellarin D, pseudostellarin F, heterophyllin A and sphinganine. This study provides experimental data to reveal the ecological environment on metabolite biosynthesis and influence of genetic variation on Pseudostellariae Radix.