Analysis of Specific Proteins in Oviductus Ranae and its Adulterants by Gel Electrophoresis Coupled LC/MS

A method for the identification of Oviductus ranae samples was established by gel electrophoresis combined with nano liquid chromatography-mass spectrometry(LC/MS). The samples including Oviductus ranae, Rana catesbeiana fallopian tube, Rana catesbeiana ovary, Rana nigromaculata ovary and walleye pollack fallopian tube were extracted by using the TCA acetone precipitation method. Comprehensive application of gel electrophoresis, enzymatic hydrolysis, nano liquid chromatography, series spectrum detection and database retrieval technology were carried on the analysis to the proteins of Oviductus ranae. At the same time, the proteins of market samples were analyzed using the same method and compared with the proteins in Oviductus ranae. The results show that the specific protein bands distribution of Oviductus ranae and the counterfeits are different, so the authenticity of Oviductus ranae samples is able to be detected by the comparation of the specific protein bands. The method is reliable for the recognition of specific proteins in Oviductus ranae.