Determination of Secoisolariciresonol Diglucoside, Secoisolariciresinol, Enterolactone and Enterodiol in Serum by LC/MS/MS

A method for determination of Secoisolariciresonol diglucoside(SDG), secoisolariciresinol(Seco), enterolactone(EL) and enterodiol(ED) in serum by LC/MS/MS was established.In this method, β-Glucuronidase was used to hydrolyze the serum samples, the electrospray ionization sourse and negative ion MRM scan were employed. The linearity correlation coefficients (r) was more than 0.999 1. The limit of quantification (LOQ, S/N=10) was 50 ng/mL for SDG, 5.0 ng/mL for Seco, 5.0 ng/mL for EL and 5.0 ng/mL for ED. The spiked recovery in blank serum sample ranged from 79.0% to 105.6% with coefficient of variation (CV, n=6) less than 19.4%. The method is sensitive and reliable, and the samples handling is simple, which is suitable for determination of SDG、Seco、EL、ED in serum sample.