Pharmacokinetics of Bifonazole in Rabbits and Quantitative Determination of Concentration in Organs

The bifonazole's plasma concentration in rabbits after bifonazole solution preparation and cream preparation spread on rabbit skin was determined,using GC/MS-SIM quantitative method,and their pharmacokinetic parameters were calculated with 3p87 software.The results showed that the plasma concentration-time curves of the two preparations in rabbits were fitted to the two-compartment open model.Tmax of solution and cream preparations were 2.84 h and 5.84 h,and Cmax,356.18ng/ml and 182.81ng/ml,respectively.The concentration of bifonazole in drug-spread skin was quantified:4200ng after one day,2500ng after two days,and 1250ng after three days respectively.However,the concentrations of bifonazole in liver,kidney,muscle,muscle beneath skin and skin for control are lower than the determination limit.