The Study of High Resolution Detection for DNA by Delayed Extraction Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

Delayed Extraction Matrix Assisted Laser Desorption/Ionization Mass Spectrometry (DE MALDI MS)was used to high resolution analysis of mix based DNA samples,using the binary matrices 3 HPA(3 Hydroxy 2 Picolinic Acid)/PA(Picolinic Acid)and substrate nitrocellose film.The results were satisfactory:the resolution of DNA 20 mer molecular ion peak was 8769,DNA 36 mer was 1650,and DNA 54 mer was 843.The molecular ion isotope peaks of 20 mer and 36 mer were separated distinctly.We also studied some kinds of factors that affect seriously the resolution of DNA mass spectra.