Preliminary Study on Direct Mass Spectrometric Analysis of Proteins Absorbed on Membrane

Cytochrome C absorbed on nitrocellulose(NC) membrane was directly analyzed by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI TOF MS).Mass accuracy and reproducibility are the same as those of standard sample preparation with the detection limitation of l pmol.Scanning electron microscopy showed that NC membrane enabled formation of protein matrix co crystalline because of its low mean pore sizes and not too deep pores.