Studies on Triterpene Saponin from Achyranthes bientata BI by Electrospray Ionization Tandem Mass Spectrometry

AchyranthosideI and Achyranthoside Ⅱ which were considered to be effective constituents from Achyranthes bientata BI were identified by electrospray ionization tandem mass spectrometric (ESI-MSn) methods after macro-porous adsorbent chromatographic separation.Their molecule masses were detected and ESI-MSn date were obtained, and fragmentation mechanisms were proposed. Their characteristic mass spectra can be use as fingerprint for the identification of AchyranthosideI and Achyranthoside Ⅱ. This method is simple, rapid and highly sensitive.