Comparison of Gas Chromatography-Mass Spectrometry Analysis in Different Extract Parts of Marchantia convoluta and Study on Partial Biologic Activity

The analysis method of different extract parts of Marchantia convoluta by gas chromatography-mass spectrometry(GC/MS) was established because of the different partial biologic activity in different parts of M. convoluta. The crude extracts of M. convoluta leaves was obtained after M. convoluta leaves were extracted with 80% ethanol and the extracts were concentrated under reduced pressure. Then the extract was partitioned using sequential extraction with organic solvents: petrol and ethyl acetate and n-butanol saturated with water. The petrol and ethyl acetate extracts were performed GC/MS analysis to identification the chemical constituents. Anti-liver cancer cells effect was studied. 54 peaks were separated and 25 compounds were identified by GC/MS from petrol extract. The main com-ponents were organic acid and ester (10). The other 7 kinds of terpenes and 8 kinds of terpenones were identified, too. 23 peaks were separated and 19 compounds were identified by GC/MS (reliability to standard MS >80%) from ethyl acetate extract. The main components were benzothianzole and cedrol (accounting for 14.97% and 20.69%). Only 4 kinds of organic acid and ester were identified from ethyl acetate extract. It was found that ethyl acetate extract induced cytotoxicity of liver cancer cells in a dose-dependent manner and 40mg/L of ethyl acetate extract can inhibited liver cancer cells evidently. Petrol extract had no effect on inhibiting liver cancer cells.