Determination of 7 Kinds of Sudan Dye in Food by HPLC-ESI-MS/MS

A method for determination of 7 kinds of Sudan dye in food by reversed phase(RP) liquid chromatography-tandem mass spectrometry coupled with electro spray (LC-ESI-MS/MS) was developed. The sample was extracted by acetonitrile and analysed by LC-ESI-MS/MS. The linear range was 5.0~30 μg/L. The average recoveries were 63.5%~106.8% and its relative standard deviation was lower than 5%. The method was sensitive, reliable, selective and reproducible.