On-target Protein Digestion of Proteins for Fast Mass Spectrometric-Peptides Mapping and Identification

ZHANG Xiang-min; YU Wen-jia; GAO Ming-xia; DENG Chun-hui; YANG Peng-yuan

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Journal of Chinese Mass Spectrometry Society > 2006 > 27(增刊): 165-166.

ZHANG Xiang-min, YU Wen-jia, GAO Ming-xia, DENG Chun-hui, YANG Peng-yuan. On-target Protein Digestion of Proteins for Fast Mass Spectrometric-Peptides Mapping and Identification[J]. Journal of Chinese Mass Spectrometry Society, 2006, 27(增刊): 165-166.

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On-target Protein Digestion of Proteins for Fast Mass Spectrometric-Peptides Mapping and Identification

Department of Chemistry, Fudan University, Shanghai 200433, China

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Corresponding author:
ZHANG Xiang-min
Received Date: December 31, 1899
Revised Date: December 31, 1899

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On-target tryptic digestion combined with MALDI-TOF/TOF was studied as a rapid and simple method for the analysis and identification of proteins. Conditions for on-target digestion optimized for temperature, diestion time, and proportion of protein and trypsin. A 15 min reaction time and at 50 ℃ and 7 100∶1 of protein vs. trypsin was finally used.

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[1]	N Lundell, T. Schreitmuller. Anal. Biochem， 1999, 266: 31.
[2]	D Ericsson, S Ekstrom, J Nilsson, et al. Proteomics， 2001, 1: 1 072.
[3]	5. Slysz G W, Schriemer D C. Anal. Chem， 2005, 77: 1 572.

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On-target Protein Digestion of Proteins for Fast Mass Spectrometric-Peptides Mapping and Identification

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