Advanced Search
ZHANG Xiang-min, YU Wen-jia, GAO Ming-xia, DENG Chun-hui, YANG Peng-yuan. On-target Protein Digestion of Proteins for Fast Mass Spectrometric-Peptides Mapping and Identification[J]. Journal of Chinese Mass Spectrometry Society, 2006, 27(增刊): 165-166.
Citation: ZHANG Xiang-min, YU Wen-jia, GAO Ming-xia, DENG Chun-hui, YANG Peng-yuan. On-target Protein Digestion of Proteins for Fast Mass Spectrometric-Peptides Mapping and Identification[J]. Journal of Chinese Mass Spectrometry Society, 2006, 27(增刊): 165-166.
shu

On-target Protein Digestion of Proteins for Fast Mass Spectrometric-Peptides Mapping and Identification

    Graphical Abstract
    • Abstract
  • On-target tryptic digestion combined with MALDI-TOF/TOF was studied as a rapid and simple method for the analysis and identification of proteins. Conditions for on-target digestion optimized for temperature, diestion time, and proportion of protein and trypsin. A 15 min reaction time and at 50 ℃ and 7 100∶1 of protein vs. trypsin was finally used.
    • FullText(HTML)
  • loading
    • References (3)
    • Related Articles

Catalog

    Turn off MathJax
    Article Contents

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return