Development of TOF-MS Used as the Detector of HPLC

JIA Wei-tao; XU Guo-bin; YAO Jun; HE Jian; ZHOU Zhen; LU Hao-jie; YANG Peng-yuan

Journal of Chinese Mass Spectrometry Society > 2006 > 27(3): 129-134.

JIA Wei-tao, XU Guo-bin, YAO Jun, HE Jian, ZHOU Zhen, LU Hao-jie, YANG Peng-yuan. Development of TOF-MS Used as the Detector of HPLC[J]. Journal of Chinese Mass Spectrometry Society, 2006, 27(3): 129-134.

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Development of TOF-MS Used as the Detector of HPLC

1.
Department of Chemistry, Fudan University, Shanghai 200433, China
2.
Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
3.
Department of Mechanical and Electrical Engineering, Xiamen University, Xiamen 361005, China
4.
Guangzhou Institute of Geochemistry Chinese Academy of Sciences, Guangzhou 510640, China

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Corresponding author:
YANG Peng-yuan
Received Date: November 27, 2005
Revised Date: January 16, 2006

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Abstract

Time-of-flight mass spectrometry (TOF-MS) is widely used in protein chemistry and proteomics research due to its high sensitivity，high resolution and high accuracy. Recently two Q-TOF-MS analytical systems are developed. Both systems are equipped with electrospray ionization source which could be directly hyphenated with HPLC as an outstanding detector. The ESI-triple quadrupoleorthogonal acceleration-reflecting TOF mass spectrometer has some promising features, such as a ESI source with heatable nebulizer settled on a smart triaxiality moving stage, an excellent ion-optics system composed with triplequadruple, double-pulse acceleration, and a double stage reflector with homemade grids. The GramicidinS sample concentration below to 3 fmol/μL can be identified by these instruments under the optimized conditions. The mass range of these instruments is from 50 u to 6 000 u with a general ESI interface to the HPLC. The optimal mass resolution exceeds 11 000 (Full Width at Half Maximum, FWHM) and the signal/noise ratio of reserpine (10 pg/μL) is greater than 100.

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