Investigation of Chemical Constituents of AntiAIV Fractions from Dryopteris Crassirhizoma by Liquid ChromatographyTandem Mass Spectrometry

The chemical constituents of antiavian influenza virus (AIV) fractions from Dryopteris Crassirhizoma were investigated using high performance liquid chromatography-tandem mass spectrometry (HPLC-MSⁿ). 52, 38 and 20 chromatogram peaks were detected using HPLC-MSⁿ in (+)ESI, (-)ESI and (+)APCI modes, respectively. The result showed that most constituents of anti-avian influenza virus (AIV) fractions from Dryopteris Crassirhizoma were phlorophenone derivatives. Fourteen phlorophenone derivatives from Dryopteris Crassirhizoma were identified and characterized basing on MSⁿ data. The results of this paper provided available data for further studies on anti AIV constituents and fingerprint of Dryopteris Crassirhizoma .