CO₂-SFE Extraction of the Lignans in Schisandra chinensis Fruits and Analysis of Extracting Products by HPLC-APCI-MS/MS

The orthogonal test was used to optimize CO₂-SFE process of the lignans in Schisandra chinensis fruits with content of total lignan as index, and the extraction products were analyzed by HPLC-APCI-MS/MS. The optimal extraction procedure is as follows: the extraction pressure is 25 MPa, the extraction temperature is 45 ℃，and the concentration alcohol is 90%. Ten structures of extraction products are confirmed by HPLC-APCI-MS/MS. The optimum extraction procedure is reasonable and stable. Compared SFE with traditional methods, the optimum extraction procedure is excellent and valuable．