UPLC/Q-TOF-MS Based the Diversity of Toxiferous Composition of the Toxicity of Animals and in Different Combination of Veratrum Nigrum and Ginseng

In order to study toxicity changes in different matching between the herbal ginseng and veratrum nigrum of eighteen incompatible medication in traditional medicine, the acute toxicity of mice treated with veratrum nigrum-ginseng combination of the eighteen incompatibal (Shi Ba Fan) was explored. UPLC/TOF-MS with Principal Components Analysis (PCA) were used to analyze the same samples used in acute toxic experiment. The results showed that death rate of mice was decreased in the rise of compatibility of ginseng with fixed dose of veratrum nigrum, toxicity alkaloid series such as veratrosine, veratrum alkamine, 3-angeloylzygadenine, germidine, veretramine, rubijervine were also increased. Therefore, above alkaloid series may be key factors contributed to the poisoning effect of ginseng combined with veratrum nigrum.