Pharmacokinetic and Metabolism Investigation of Hodroxylated PBDE Congeners in Rat

Ultra performance liquid chromatography (UPLC) provides improved resolution, speed and sensitivity compared to conventional HPLC. In this study, a robust UPLC-APCI-MS method was developed for the determination of nine OH-PBDE congeners in rat plasma. Under the optimized conditions, the OH-PBDE congeners were eluted within 7.0 min. The limits of quantification defined at S/N of 10 was 0.4-3.0 μg•L－1 in rat plasma. The method provided good linearity for the calibration curves with recoveries of 83.8%-112.9% and repeatability of 0.8%-7.4% for intra-day and 3.5%-14.1% for inter-day measurements. The developed method was applied for supporting the pharmacokinetics investigation of 6-OH-BDE-47 in two groups of S. D. rats that received respectively a single dose of 0.60 mg•kg－1 (high dose) and 0.15 mg•kg－1 (low dose) by intravenous injection. The results showed that plasma levels of 6-OH-BDE-47 declined bi-exponentially, with mean elimination half-life of 71.7 and 85.6 min for lower and higher group respectively. The obtained results of short elimination half-life suggested that 6-OH-BDE-47 might not accumulate significantly in rat.