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WEI Jun-ying, ZHANG Yang-jun, SUN Ai-hua, ZHAO Yan, DAI Jie, YING Wan-tao, HAO Yun-wei, JIANG Ying, QIAN Xiao-hong. Analysis of the Protein Subcellular Localization by Multiple Reaction Monitoring-Mass Spectrometry (MRM-MS)[J]. Journal of Chinese Mass Spectrometry Society, 2010, 31(增刊): 16-16.
Citation: WEI Jun-ying, ZHANG Yang-jun, SUN Ai-hua, ZHAO Yan, DAI Jie, YING Wan-tao, HAO Yun-wei, JIANG Ying, QIAN Xiao-hong. Analysis of the Protein Subcellular Localization by Multiple Reaction Monitoring-Mass Spectrometry (MRM-MS)[J]. Journal of Chinese Mass Spectrometry Society, 2010, 31(增刊): 16-16.
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Analysis of the Protein Subcellular Localization by Multiple Reaction Monitoring-Mass Spectrometry (MRM-MS)

  • 1.

    Beijing Institute of Technology, Beijing 100081, China

  • 2.

    Academy of Military Medical Science, Beijing 102206, China

  • 3.

    Thermo Fisher Scientific(Shanghai) co., Ltd, Shanghai 201206, China

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  • Received Date: December 31, 1899
  • Revised Date: December 31, 1899
    Graphical Abstract
    • Abstract
  • The subcellular localization of a protein, as well as its quantity, can provide important information about its function within the cell. Many experimental methods, such as green fluorescent protein based assay and organelle isolation based proteomics strategy, have been used to map proteins to their intracellular locations. In this study, a new approach based on multiple reaction monitoring-mass spectrometry (MRM-MS) was introduced to simultaneously assay the subcellular localization of a protein and its quantity within the organelle.
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    • References (3)
  • [1]
    BERGERON J J M, et al. Quantitative proteomics analysis of the secretory pathway[J]. Cell, 2006, 127: 1 265-1 281.
    [2]
    AEBERSOLD R, et al. Proteome-wide cellular protein concentrations of the human pathogen Leptospira interrogans[J]. Nature, 2009, 460: 762-765.
    [3]
    SONG Y P, et al. Sample preparation project for the subcellular proteome of mouse liver. Proteomics[J]. 2006, 6(19): 5 269-5 277.
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