Analysis for Separation Products of Gentiana Scabra Bunge through High Speed Counter Current Chromatography by Electrospray Mass Spectrometry

High speed counter current chromatography was successfully applied to the preparative separation of the component of Gentiana scabra Bunge. The separation fraction was identified by ESI-MSn included Gentiopicrin and sweroside. The purity of Gentiopicrin and sweroside were over 96.5% and 74.5% via HPLC, respectively.