Peptide Mapping and Primary Structural Analysis of Cytochrome C by Liquid Chromatography-Mass Spectrometry

Liquid chromagraphy-mass spectrometry (LC/MS)was used to analyze the peptide mapping of Cyt. C digested by TPCK-trypsin for determination of the molecular weight of each peptide fragment. Most of the peptide fragments were sequenced by tandem MS (MS/MS)to obtain the amino acid sequence. The whole method of liquid chromagraphy -mass spectrometry(LC/MS)has been founded and applied to biological molecules.