Qualitative Analysis of Intact Glucosinolates by High Performance Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry

The retention times and negative mass spectra of intact glacosinolates extracted from the kale and tai tsai and the reference samples are measured by high peformance liquid chromatography-electrospray ionization tandem mass spectrometry(HPLC-ESI/MS n,n=1～2), respectively. The unknown chromatography peaks in eight species of glucosinolates were certified by the sqnare peaks to normalization. Negative quasimolecular ion mass spectra were observed. Daughter ions at m/z 75 and m/z 97 present in the spectra of all the GS investigated and its are - and - moieties, respectively. Ions R-(C=NOH)-S -, which provides side chain information, were also observed.