Analysis of Endocrine Disruptors in Water by Solid Phase Extraction-Liquid Chromatography-Ion Trap Mass/Mass Spectrometry

A sensitive method was developed for the analysis of three endocrine-disrupting compounds including bisphenol A (BPA), 17β-estradiol (E2), and 17α-ethynylestradiol (EE2) using liquid chromatography-ion trap mass/mass spectrometry (LC-MS/MS) coupled with solid phase extraction (SPE). The results showed that the MS/MS spectra of BPA and E2 are the same as the results reported, while the MS/MS spectra of EE2 is different from that presented in the literatures. The quantitative analysis for BPA, E2, and EE2 were conducted using the fragment ions of m/z 212, m/z 145 and m/z 183, and m/z 267, respectively. The detection limits of 8μg/L for BPA, 28μg/L for E2, and 16μg/L for EE2 by (LC-MS/MS) were achieved. The concentration of common ions and natural organic matters and pH in drinking water, surface water, groundwater samples have effects on recoveries of selected endocrine disruptors, and recoveries (r) of BPA, E2, and EE2 are in the order of r(de-ionized water) > r(underground water) > r(surface water).