Applications of Organic Mass Spectrometry in Structure Identification of an Impurity Compound in Prepared Mangiferin Extracted from Mangifera indica L. leaves

An impurity compound in prepared mangiferin extracted from Mangifera indica L. leaves was isolated by high performance liquid chromatographic (HPLC). Its structure was identified by HR-ESI (-)-MS/MS and further confirmed by NMR spectroscopic studies. Based on the spectral data, the molecular structure of the impurity is identified as 2-C-β-D-glucopyranosyl-1, 6, 7-trihydroxy-3-methoxy-xanthen-9-one. The fragmentation behaviors of mangiferin and the impurity are proposed.