Simultaneous Determination of 10 Anabolic Steroids Contaminations in Milk by Liquid Chromatography-Tandem Mass Spectrometry

A liquid chromatographic/tandem mass spectrometric (LC/MS/MS) multiresidue method for the simultaneous determination of 10 anabolic steroids (ASs) in whole milk were developed. Milk samples were extracted with methanol and the samples were then subjected to a clean-up procedure using liquid-liquid extraction (LLE) methods. The samples were analysed by LC/MS/MS. The limits of detection (LOD) of LC/MS/MS method used for testing the 10 ASs in whole milk ranged from 0.06 to 0.22 µg•L^－1, and the limits of quantification (LOQ) were from 0.12 to 0.54 µg•L^－1. Experiments on spiked samples of whole milk showed that at addition levels of 1.0, 2.0, 10 µg•L^－1 the average recoveries of the ASs were 24% to 86%, 25% to 91%, 31% to 71%, with relative standard deviations (RSDs) ranged from 12% to 37%, 5% to 20%, 10% to 16%, respectively.