Analysis of Diterpene Quinones in Danshen ( Salvia Miltiorrhiza Bunge ) by HPLC-ESI/TOFMS
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Abstract
The diterpene quinone components in lipid soluble fractions of Danshen were studied by HPLC-ESI/TOFMS. The lipid solubles were extracted with a mixture of chloroform and methanol (1∶4, V/V). The extract was dried by a gentle stream of nitrogen, and the residue was diluted in methanol for analysis. HPLC was performed on a reversed-phase C18 column using isocratic elution with a mixture of methanol and water with the addition of 0.1% acetic acid and 5 mmol•L-1 ammonium acetate. Accurate molecular weights of individual tanshinones were determined through ESI-TOF/MS on-line detection. The molecular formula and TOFMS fragmentation behavior of each compound were obtained through off-line analysis under different collision induced dissociation (CID) energies. Combining literature information with the observed data, 16 tanshinones in lipid-soluble fractions of Danshen were tentatively identified in a run. The method demonstrates high potential as a rapid and effective method for the preliminary structural screening and speciation of bioactive tanshinone components in Danshen or other herbal extracts.
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