Direct Analysis of Metabolites of Aconitine under Rat Intestinal Bacteria by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

WANG Xi-ye; LIU Wen-long; PI Zi-feng; LIU Shu-ying

Journal of Chinese Mass Spectrometry Society > 2009 > 30(增刊): 120-121.

WANG Xi-ye, LIU Wen-long, PI Zi-feng, LIU Shu-ying. Direct Analysis of Metabolites of Aconitine under Rat Intestinal Bacteria by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 2009, 30(增刊): 120-121.

Citation:

PDF (533 KB)

Direct Analysis of Metabolites of Aconitine under Rat Intestinal Bacteria by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

1.
Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
2.
Graduate School of the Chinese Academy of Sciences, Beijing 100039, China

More Information

Received Date: December 31, 1899
Revised Date: December 31, 1899

Graphical Abstract

Abstract

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry was used to direct analyze the metabolites of aconitine under rat intestinal bacteria. Only simple sample preparation was needed prior to the MS experiment. CHCA given the good signals was screened as the matrix for the analysis of metabolites. Aconitine can be converted into its metabolites by rat intestinal bacteria such as monoester-diterpenoid alkaloids, diester-diterpenoid alkaloids and lipo-alkaloids by deacetylation, dehydroxylation, demethylation and esterification.

FullText(HTML)

References (3)

References

[1]	赵宇峰, 刘淑莹, 等. 利用软电离质谱技术研究乌头碱在肠内细菌中的生物转化[J]. 高等学校化学学报, 2008, 29(1): 55-59.
[2]	KAWATA Y, MESELHY M R, NAKAMURA N, et al. Conversion of aconitine to lipoaconitine by human intestinal bacteria and their antinociceptive effects in mice[J]. Journal of traditional medicines, 1999, 16: 15-23.
[3]	WU W, LIANG Z T, ZHAO Z Z, et al. Direct analysis of alkaloid profiling in plant tissue by using matrix-assisted laser desorption/ionization mass spectrometry[J]. Journal of mass spectrometry, 2007, 42(1): 58-69.

Cited By

Get Citation

PDF

XML

Article views (319) PDF downloads (942)

Turn off MathJax

Article Contents

Abstract

References

Direct Analysis of Metabolites of Aconitine under Rat Intestinal Bacteria by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Abstract

References

Catalog

Export File

Citation

Format

Content