Effect of Auxiliary Electrospray on Liquid Chromatography-Electrospray Ionization-Mass Spectrometry

CHEN Ming; WANG Li-qun; GAO Xiu-jie; GONG Jing-bo; QIAN Xiao-hong; QIAN Ling-jia

Journal of Chinese Mass Spectrometry Society > 2009 > 30(6): 321-326.

CHEN Ming, WANG Li-qun, GAO Xiu-jie, GONG Jing-bo, QIAN Xiao-hong, QIAN Ling-jia. Effect of Auxiliary Electrospray on Liquid Chromatography-Electrospray Ionization-Mass Spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 2009, 30(6): 321-326.

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Effect of Auxiliary Electrospray on Liquid Chromatography-Electrospray Ionization-Mass Spectrometry

1.
Institute of Hygiene and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China
2.
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China

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Received Date: December 31, 1899
Revised Date: December 31, 1899

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Abstract

The efficiency of electrospray ionization was affected by the component of solvent. The effect of different concentration of ACN on mass spectrometry for MRFA, Reserpine, ΜLtramark1621 and peptide mixture were observed, and found that all of the chemicals have the max mass spectra signal intensity in 70% ACN. Then, the peptide mixtures from myoglobin were analyzed using CapLC-ESI-LTQ system， which were equipped with auxiliary electrospray by 100%ACN, 0.1%FA. This results show that the detection ratio of peptides and the recovery of protein sequence are remarkable improved.
- electrospray ionization (ESI),
- peptide,
- mass spectrometry

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