Determination of Carbamate Pesticides and Kill Silkworm Toxin in Human Blood Using Microwave Extraction-PTV-GC/MS with Gel Permeation Chromatography Clean-Up

Carbamate pesticides and kill silkworm toxin in human blood were analyzed by microwave extraction-PTV-GC/MS and gel permeation chromatography (GPC) clean-up. The results showed that pesticide residues were extracted from blood with solvents by microwave assisted extraction, extracts were cleaned up by GPC, and analyzed by gas chromatography with mass selectived etectionin sis model. Programmed temperature vaporization(PTV) large volume injector(LVI)was applied to inject the sample. The rates of recovery range from 68% to 91% . The detection limits of pesticides are 0.001-0.47 mg•L^-1. The method can be used for simultaneous determination of pesticide residues in blood. It is sensitive and simple， and meets the determinative requirement of pesticide residues.