Screening and Structures Characterization of α-Glucosidase Inhibitors from Hawthorn Leaves Flavonoids Extract by Ultrafiltration LC-MSⁿ

In vitro α-glycosidase inhibition assays and Ultrafiltration LC-DAD-ESI-MSⁿ were combined to screening α-glucosidase inhibitors from hawthorn leaves flavonoids extract. As a result, hawthorn leaves flavonoids extract showed strong α-glucosidase inhibitory activity, four compounds presented α-glucosidase inhibitory effects were observed and identified by LC-DAD-MSⁿ, and further confirmed by high resolution SORI-CID FT ICR MS data.