Analysis of Anisodamine Derivatives by Electrospray Ionization-Ion Trap Mass Spectrometry

Anisodamine shows activity as cholinoceptor blocking agent. Some new anisodamine derivatives were synthesized. To confirm the structures of those derivatives, their mass spectrometries were studied through electrospray ionization-ion trap mass spectrometer. The results indicated that anisodamine derivatives modified by benzyl, the quaternary ammonium chloride, could form a molecular-related ion M+35- and producted ions through elimination of methyl chloride or benzyl chloride in spectra of negative ion mode. In positive ion mode, ESI-MS spectra of three compounds all gave molecular-related ions M-35+. Their MS 2-4spectra gave the characteristic product ions which were also formed by the cleavage of C-N bond accompanied with breaking of HO-C, H-C or RCOO-C bonds of the quaternary ammonium cation of compound a-c.