Differentiation of Ginsenoside Isomers Rh<sub>2</sub> and CK Using ESI-Q-TOF-MS/MS

SUN Jing-hui; WU Wei; GUO Ying-ying; QIN Qiu-jie; LIU Shu-ying

doi:10.7538/zpxb.2014.35.02.0158

Journal of Chinese Mass Spectrometry Society > 2014 > 35(2): 158-162. > DOI: 10.7538/zpxb.2014.35.02.0158 CSTR: 32365.14.zpxb.2014.35.02.0158

SUN Jing-hui, WU Wei, GUO Ying-ying, QIN Qiu-jie, LIU Shu-ying. Differentiation of Ginsenoside Isomers Rh₂ and CK Using ESI-Q-TOF-MS/MS[J]. Journal of Chinese Mass Spectrometry Society, 2014, 35(2): 158-162. DOI: 10.7538/zpxb.2014.35.02.0158

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Differentiation of Ginsenoside Isomers Rh₂ and CK Using ESI-Q-TOF-MS/MS

1.
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China
2.
Pharmaceutical College, Beihua University, Jilin 132013, China
3.
Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

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Abstract

Abstract

A rapid analysis method of electrospray-quadrupole-time-of-flight mass spectrometry (ESI-Q-TOF-MS/MS) was established to differentiate ginsenoside isomers Rh₂ and compound K (CK). Based on the analysis of MS/MS product ions with different collision energy in both negative and positive ion mode, ginsenoside isomers Rh₂ and CK are distinguished. The results show that the fragmentation pathway of Rh₂ and CK in negative ion mode is similar. The difference is that the fragment ion abundance of Rh₂ is significantly lower than that of CK with the same collision energy. The fragmentation behavior of Rh₂ is different from CK in positive ion mode, specific fragment ions m/z 465 and m/z 203 are produced with collision energy 20, 40 and 60 eV in CK, but Rh₂ produces the similar specific fragment ions only with collision energy 60 eV in MS/MS. The MS/MS fragmentation pathway is particularly useful in the identification of ginsenoside isomers Rh₂and CK.
- electrospray-quadrupole-time-of-flight mass spectrometry (ESI-Q-TOF-MS/MS）,
- ginsenoside,
- isomer

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References

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Differentiation of Ginsenoside Isomers Rh₂ and CK Using ESI-Q-TOF-MS/MS

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Differentiation of Ginsenoside Isomers Rh₂ and CK Using ESI-Q-TOF-MS/MS