Determination of Seven Microcystins in Aquatic Products by Liquid Chromatography Tandem Mass Spectrometry

YANG Zhen-yu; ZHOU Yao

doi:10.7538/zpxb.youxian.2014.0035

Journal of Chinese Mass Spectrometry Society > 2014 > 35(5): 447-453. > DOI: 10.7538/zpxb.youxian.2014.0035 CSTR: 32365.14.zpxb.youxian.2014.0035

YANG Zhen-yu, ZHOU Yao. Determination of Seven Microcystins in Aquatic Products by Liquid Chromatography Tandem Mass Spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 2014, 35(5): 447-453. DOI: 10.7538/zpxb.youxian.2014.0035

Citation:

PDF (649 KB)

Determination of Seven Microcystins in Aquatic Products by Liquid Chromatography Tandem Mass Spectrometry

Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China

More Information

Graphical Abstract

Abstract

Abstract

Microcystins are common hepatotoxic cyclic toxins released from blue and green algae, which can harm to animal and human health．It is important to establish an accurate and rapid method for the safety of aquatic products. All the parameters of sample preparation and instrumental methods were optimized. Seven microcystins (LR, RR, YR, LW, LA, LF, and LY) in aquatic products were determined simultaneously by liquid chrometography tandem mass spectrometry. Solid-phase extraction(SPE) and matrix solid-phase extraction (MSPD) were adopted for sample preparation. For SPE method, the LOQ is 0.5 μg/kg, and the recoveries are between 75% and 106% with RSD less than 20%. For MSPD method, the LOQ is 2.5 μg/kg, and the recoveries are between 73% and 93% with RSD less than 16%. This method is suitable for daily inspection and confirmation of microcystins in aquatic products.

FullText(HTML)

References (22)

References

[1]	李效宇. 微囊藻毒素及其毒理学研究[M]. 北京:科学出版社,2007.
[2]	谢平. 水生动物体内的微囊藻毒素及其对人类健康的潜在威胁[M]. 北京:科学出版社,2006.
[3]	张敬平, 肖付刚, 赵晓联. 微囊藻毒素分析检测技术[M]. 北京:化学工业出版社,2010.
[4]	MORENO I M，MOLINA R， JOS A， et al. Determination of microcystins in fish by solvent extraction and liquid chromatography[J]. J Chromatogr A, 2005,1 080（2）:199-203.
[5]	虞锐鹏, 陶冠军, 贡小清,等. 高效液相色谱-质谱联用方法测定背角无齿蚌体内微囊藻毒素[J]. 分析测试学报, 2007,26(5):671-674.YU Ruipeng, TAO Guanjun, GONG Xiaoqing, et al. Determination of microcystins in anodonta woodiana by high performance liquid chromatography combined with electrospray ionization mass spectrometry[J]. Journal of Instrumental Analysis, 2007, 26(5):671-674(in Chinese).
[6]	MEKEBRI A, BLONDINA G J, CRANE D B. Method validation of microcystins in water and tissue by enhanced liquid chromatography tandem mass spectrometry[J]. J Chromatogr A, 2009,1216（15）:3147-3155.
[7]	刘晓蕙. 多聚酶链反应(PCR)技术监测水中微囊藻毒素的方法及进展[J]. 河南预防医学杂志，2004,15(6):365-367.LIU Xiaohui. Method and progress of determinate the microcystins in water by PCR technical[J]. Henan J Prev Med, 2004,15(6):365-367(in Chinese).
[8]	闫建秀, 虞锐鹏, 汤坚, 等. 固相萃取-反相高效液相色谱法检测太湖蛳螺中微囊藻毒素[J]. 食品与机械,2008, 24(5):92-95.YAN Jianxiu, YU Ruipeng, TANG Jian, et al. Determination of microcystins in the snail genus eriocheir from Taihu using RP-HPLC with an efficient solid phase extraction[J]. Food & Machinery,2008, 24(5):92-95(in Chinese).
[9]	王蕾，李小艳，薛文通,等. 微囊藻毒素检测方法研究进展[J]. 食品科学,2005,26（增刊）:135-138.WANG Lei, LI Xiaoyan, XUE Wentong,et al. Review on research progress of microcystins detection[J]. Food Science, 2005, 26(Suppl):135-138(in Chinese).
[10]	赵晓联，孙秀兰，汤坚. 藻毒素的危害及分析方法进展[J]. 食品科学,2005, 26(3):257-261.ZHAO Xiaolian, SUN Xiulan, TANG Jian. Development of assay methods and hazards of microcystins[J]. Food Science, 2005, 26(3):257-261(in Chinese).
[11]	BRUNO M, FIORI M, MATTEI D, et al. ELISA and LC-MS/MS methods for determining cyanobacterial toxins in blue-green algae food supplements[J]. Natural Product Research, 2006， 9（20）： 827-834.
[12]	DAI M, XIE P, CHEN J, et al. Quantitative determination of microcystins in rat plasma by LC-ESI tandem MS[J]. Chromatographia, 2008, 68（9/10）： 811-815.
[13]	虞锐鹏, 陶冠军, 杨健, 等. 超高效液相色谱-四极杆-飞行时间质谱法快速测定水产品中微囊藻毒素和节球藻毒素[J]. 分析试验室,2012,31(1):80-83.YU Ruipeng, TAO Guanjun, YANG Jian, et al. Determination of microcystins and nodularins in aquatic products by ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry[J]. Chinese Journal of Analysis Laboratory, 2012, 31(1)：80-83(in Chinese).
[14]	明俊超, 姜海洲, 袁新华. 微囊藻毒素对鱼类的毒性效应及其作用机理研究进展[J]. 中国农学通报, 2012,28(35):69-74.Ming Junchao, Jiang Haizhou, Yuan Xinhua. A review of microcystin of toxic effects and mechanisms in exposed fish[J]. Chinese Agricultural Science Bulletin, 2012,28(35):69-74(in Chinese).
[15]	WELLER M G. Immunoassays and biosensors for the detection of cyanobacterial toxins in water[J]. Sensors, 2013, （13）： 15085-15112.
[16]	XU W, CHEN Q, ZHANG T, et al. Development and application of ultra performance liquid chromatography-electrospray ionization tandem triple quadrupole mass spectrometry for determination of seven microcystins in water samples[J]. Analytica Chimica Acta, 2008， 626（1）：28-36.
[17]	DAI M, XIE P, LIANG G D, et al. Simultaneous determination of microcystin-LR and its glutathione conjugate in fish tissues by liquid chromatography-tandem mass spectrometry[J]. Journal of Chromatography B, 2008， 862（1/2):43-50.
[18]	钮伟民，肖付刚，戴维杰, 等. 液相色谱-串联质谱法同时检测水中5种微囊藻毒素[J]. 安徽农业科学, 2008，36(29):12554-12556.NIU Weimin, XIAO Fugang, DAI Weijie, et al. Simultaneous deterndnation of nve microcystins in water by mgh perfornmnce liquid chromatography-tandem mass spectrometry [J]. Journal of Anhui Agri Sci, 2008，36(29):12554-12556(in Chinese).
[19]	李尧, 张雪梅, 党献民, 等. 基质分散固相萃取净化液相色谱检测谷物中赭曲霉毒素A[J]. 粮食与饲料, 2012,(10):57-60.LI Yao, ZHANG Xuemei, DANG Xianmin, et al. Determination of ochratoxin A in cereal by high performance liquid chromatography with matrix solid-phase dispersion cleaning [J]. Cereal & Feed industry, 2012,(10):57-60 (in Chinese).
[20]	乔凤霞, 孙汉文, 刘广宇, 等. 基质固相分散-牛奶、蜂蜜中喹诺酮的多残留分析[J]. 河北大学学报：自然科学版,2008, 28(6):620-624.QIAO Fengxia, SUN Hanwen, LIU Guangyu, et al. Matrix solid-phase dispersion for multi-residues analysis of quinolones in milk and honey [J]. Journal of Hebei University(Natural Science Edition), 2008, 28(6):620-624(in Chinese).
[21]	CAMEN A, MORENO I M, RUIZ M J， et al. Determination of microcystins in natural blooms and cyanobacterial strain cultures by matrix solid-phase dispersion and liquid chromatography-mass spectrometry[J]. Anal Bioanal Chem, 2004, 380（3）:537-544.
[22]	RUIZ M J, CAME N A M， MORENO I M， et al.Determination of microcystins in biological samples by matrix solid-phase dispersion and liquid chromatography-mass spectrometry[J]. Journal of Chromatography A, 2005，1073（1/2):257-262.

[1]	YANG Yang, REN Jie-fang, TANG Sha-li, CAO Hui, ZHOU Xue-jun, WANG Jin. Determination of 25 Organophosphate Flame Retardants in Polyurethane Products by Dispersive Solid Phase Extraction and Liquid Chromatography-Tandem Mass Spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 2025, 46(2): 206-216. DOI: 10.7538/zpxb.2024.0091
[2]	NI Zheng-fei, GU Ye, FENG Yong-wei, XUE Qing-hai, WANG Qin. Simultaneous Determination of 4 Eugenol Derivatives Anesthetics Residues in Aquatic Products by UPLC-MS/MS[J]. Journal of Chinese Mass Spectrometry Society, 2018, 39(4): 451-458. DOI: 10.7538/zpxb.2017.0160
[3]	SUN Peng, GAO Yu-ling, WANG Jin-mei, LIU Xiu-juan. Determination of Eugenol Residue in Aquatic Using Dispersive Liquid-Liquid Microextraction Based on the Solidification of a Floating Organic Droplet Followed by UHPLC-MS/MS[J]. Journal of Chinese Mass Spectrometry Society, 2017, 38(3): 342-348. DOI: 10.7538/zpxb.2016.0091
[4]	ZHANG Hua-wei, LIU Hui-hui, TIAN Xiu-hui, DENG Xu-xiu, HUANG Hui, HAN Dian-feng, XU Ying-jiang, GONG Xiang-hong. Determination of 9 Triazine Herbicides in Aquatic Products by GPC-SPE-GC-MS/MS[J]. Journal of Chinese Mass Spectrometry Society, 2015, 36(2): 177-184. DOI: 10.7538/zpxb.youxian.2014.0054
[5]	YU Hui-juan, ZHANG Shan-shan, ZHANG Xiao-ling, HUI Yun-hua, GU Run-run, FENG Bing. Simultaneous Determination of the Estrogen, Progestogen and Androgen in Aquatic Products by Isotope Dilution LC-MS/MS[J]. Journal of Chinese Mass Spectrometry Society, 2014, 35(2): 170-178. DOI: 10.7538/zpxb.2014.35.02.0170
[6]	LV Qing, LI Hai-yu, LI Pi, LI Wen-tao, GUO Xiang-yu, WANG Ye, BAI Hua, QI Mei-ling, ZHANG Qing. Determination of 10 Preservatives in Wooden Children's Products by Gas Chromatography-Tandem Mass Spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 2013, 34(6): 321-329. DOI: 10.7538/zpxb.2013.34.06.0321
[7]	LI Qiang, XIA Jing, BAI Yan-kun, CAI Li-peng, ZHANG Yan. Determination of 17 Kinds of Polychlorinated Biphenyls(PCBs) in Fishery Product by MAE-SPE-GC-MS/MS[J]. Journal of Chinese Mass Spectrometry Society, 2012, 33(5): 295-300.
[8]	XU Ying-jiang, ZHANG Shi-juan, GONG Xiang-hong, LIU Hui-hui, DENG Xu-xiu, ZHANG Hua-wei, ZHANG Xiu-zhen. Determination of Bromophenols in Fishery Product by GPC and GC/MS[J]. Journal of Chinese Mass Spectrometry Society, 2011, 32(6): 350-354.
[9]	ZHANG Hai-qi, LIANG Li-jun, HE Zhong-yang, SHI Li-ke. Determination of Auramine O in Fishery Products by High-Performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 2010, 31(1): 48-51.
[10]	ZHANG Zhi-gang, SHI Bing, WANG Gen-fang, LIN Li-yi, ZHOU Yu. Determination of Medroxyprogesterone Acetate in Aquatic Product by Liquid Chromatography with Tandem Mass Spectrometric Detection[J]. Journal of Chinese Mass Spectrometry Society, 2006, 27(1): 36-39.

Cited By

Get Citation

PDF

XML

Article views (933) PDF downloads (1398)

Turn off MathJax

Article Contents

Abstract

References

Determination of Seven Microcystins in Aquatic Products by Liquid Chromatography Tandem Mass Spectrometry

Abstract

References

Related Articles

Catalog

Determination of Seven Microcystins in Aquatic Products by Liquid Chromatography Tandem Mass Spectrometry

Abstract

References

Related Articles

Catalog

Export File

Citation

Format

Content