液相色谱-串联质谱法检测动物源性水产品中7种微囊藻毒素

杨振宇; 周瑶

doi:10.7538/zpxb.youxian.2014.0035

液相色谱-串联质谱法检测动物源性水产品中7种微囊藻毒素

杨振宇,
周瑶

上海出入境检验检疫局,上海200135

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- 刊出日期: 2014-09-19

Determination of Seven Microcystins in Aquatic Products by Liquid Chromatography Tandem Mass Spectrometry

Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China

摘要

摘要: 微囊藻毒素是由淡水蓝绿藻产生的一类最常见的环肽肝毒素，对动物及人类健康具有较大的危害性，建立准确、快速的检测微囊藻毒素的方法对于水产品食用安全非常重要。本实验对动物源水产品样品的提取、固相萃取、色谱和质谱等条件进行优化，建立了液相色谱-串联质谱同时检测7种微囊藻毒素（LR、RR、YR、LW、LA、LF、LY）的方法，并采用了固相萃取和基质分散固相萃取两种前处理方法。固相萃取方法的检出限为0.5 μg/kg，添加回收率为75%～106%，相对标准偏差小于20%；基质分散固相萃取方法的检出限为2.5 μg/kg，添加回收率为73%～93%，相对标准偏差小于16%。该方法适用于水产品中微囊藻毒素的日常检测和确证。
- 微囊藻毒素 /
- 液相色谱-串联质谱 /
- 固相萃取 /
- 基质分散固相萃取 /
- 水产品
Abstract: Microcystins are common hepatotoxic cyclic toxins released from blue and green algae, which can harm to animal and human health．It is important to establish an accurate and rapid method for the safety of aquatic products. All the parameters of sample preparation and instrumental methods were optimized. Seven microcystins (LR, RR, YR, LW, LA, LF, and LY) in aquatic products were determined simultaneously by liquid chrometography tandem mass spectrometry. Solid-phase extraction(SPE) and matrix solid-phase extraction (MSPD) were adopted for sample preparation. For SPE method, the LOQ is 0.5 μg/kg, and the recoveries are between 75% and 106% with RSD less than 20%. For MSPD method, the LOQ is 2.5 μg/kg, and the recoveries are between 73% and 93% with RSD less than 16%. This method is suitable for daily inspection and confirmation of microcystins in aquatic products.
- microcystins /
- liquid chromatography tandem mass spectrometry /
- solid-phase extraction /
- matrix solid-phase extraction /
- aquatic products

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参考文献(22)

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液相色谱-串联质谱法检测动物源性水产品中7种微囊藻毒素

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Determination of Seven Microcystins in Aquatic Products by Liquid Chromatography Tandem Mass Spectrometry

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