RRLC-Q-TOF MS/MS法分析生晒参和大力参中的皂苷类成分

郝颖, 于珊珊, 戴雨霖, 张颖, 钟薇, 刘淑莹, 越皓

郝颖, 于珊珊, 戴雨霖, 张颖, 钟薇, 刘淑莹, 越皓. RRLC-Q-TOF MS/MS法分析生晒参和大力参中的皂苷类成分[J]. 质谱学报, 2014, 35(4): 311-316. DOI: 10.7538/zpxb.youxian.2014.0037
引用本文: 郝颖, 于珊珊, 戴雨霖, 张颖, 钟薇, 刘淑莹, 越皓. RRLC-Q-TOF MS/MS法分析生晒参和大力参中的皂苷类成分[J]. 质谱学报, 2014, 35(4): 311-316. DOI: 10.7538/zpxb.youxian.2014.0037
HAO Ying, YU Shan-shan, DAI Yu-lin, ZHANG Ying, ZHONG Wei, LIU Shu-ying, YUE Hao. Study on Ginsenosides in White Ginseng and Dali Ginseng by RRLC-Q-TOF MS/MS[J]. Journal of Chinese Mass Spectrometry Society, 2014, 35(4): 311-316. DOI: 10.7538/zpxb.youxian.2014.0037
Citation: HAO Ying, YU Shan-shan, DAI Yu-lin, ZHANG Ying, ZHONG Wei, LIU Shu-ying, YUE Hao. Study on Ginsenosides in White Ginseng and Dali Ginseng by RRLC-Q-TOF MS/MS[J]. Journal of Chinese Mass Spectrometry Society, 2014, 35(4): 311-316. DOI: 10.7538/zpxb.youxian.2014.0037
郝颖, 于珊珊, 戴雨霖, 张颖, 钟薇, 刘淑莹, 越皓. RRLC-Q-TOF MS/MS法分析生晒参和大力参中的皂苷类成分[J]. 质谱学报, 2014, 35(4): 311-316. CSTR: 32365.14.zpxb.youxian.2014.0037
引用本文: 郝颖, 于珊珊, 戴雨霖, 张颖, 钟薇, 刘淑莹, 越皓. RRLC-Q-TOF MS/MS法分析生晒参和大力参中的皂苷类成分[J]. 质谱学报, 2014, 35(4): 311-316. CSTR: 32365.14.zpxb.youxian.2014.0037
HAO Ying, YU Shan-shan, DAI Yu-lin, ZHANG Ying, ZHONG Wei, LIU Shu-ying, YUE Hao. Study on Ginsenosides in White Ginseng and Dali Ginseng by RRLC-Q-TOF MS/MS[J]. Journal of Chinese Mass Spectrometry Society, 2014, 35(4): 311-316. CSTR: 32365.14.zpxb.youxian.2014.0037
Citation: HAO Ying, YU Shan-shan, DAI Yu-lin, ZHANG Ying, ZHONG Wei, LIU Shu-ying, YUE Hao. Study on Ginsenosides in White Ginseng and Dali Ginseng by RRLC-Q-TOF MS/MS[J]. Journal of Chinese Mass Spectrometry Society, 2014, 35(4): 311-316. CSTR: 32365.14.zpxb.youxian.2014.0037

RRLC-Q-TOF MS/MS法分析生晒参和大力参中的皂苷类成分

Study on Ginsenosides in White Ginseng and Dali Ginseng by RRLC-Q-TOF MS/MS

  • 摘要: 利用高分离度快速液相色谱与四极杆-飞行时间质谱(RRLC-Q-TOF MS/MS)联用,对生晒参和大力参中的人参皂苷类成分进行比较研究。基于人参皂苷Rd和Re、Rf和Rg1两组同分异构体在串联质谱中的裂解规律,以及mRd的串联质谱特征,通过标准品保留时间和精确分子质量鉴定了人参中的35种人参皂苷,进而比较了生晒参和大力参中人参皂苷的相对百分含量差异,并于大力参中检测到稀有人参皂苷,如人参皂苷Rg5、Rk1、F4、Rg6 等。该方法对于大力参类传统中药炮制品的成分研究具有借鉴意义,研究结果能说明大力参区别于生晒参的物质基础,可以为大力参在临床上合理、有效地使用提供指导。
    Abstract: Rapid resolution liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry (RRLC-Q-TOF MS/MS) was used to compare the white ginseng with dali ginseng in the composition of ginsenosides. Based on the fragmentation law of the isomeric compounds, ginsenoside Rd and Re, ginsenoside Rf and Rg1, and the feature of ginsenoside mRd in MS/MS, 35 kinds of ginsenosides were identified by the comparison of the retention time of the standard compound and the accurate mass obtained from RRLC-Q-TOF MS/MS. The rare ginsenosides were detected by the comparison of white ginseng with dali ginseng, such as ginsenoside Rg5, Rk1, F4, Rg6 etc. The methods has reference significance for the research of compounds in the processed products of herbs, like dali ginseng. The results provide the reference for clinical rational drug use.
  • [1] 王悦虹, 娄大伟, 于晓洋, 等. 人参的药理学作用研究进展[J]. 吉林化工学院学报, 2010, 27(2): 38-41.WANG Yuehong, LOU Dawei, YU Xiaoyang, et al. Recent adwances in pharmacological effects of ginseng[J].Journal of Jilin Institute of Chemical Technology, 2010, 27(2): 38-41(in Chinese).
    [2] 曹智, 张燕娣, 许永华, 等. 人参有效成分及其药理作用研究新进展[J]. 人参研究, 2012, (2): 39-43.CAO Zhi, ZHANG Yandi, XU Yonghua, et al. Active ingredients and pharmacological activities of ginseng new progress [J]. Ginseng Research, 2012, (2): 39-43(in Chinese).
    [3] 张萍,张南平,肖新月, 等. 人参皂苷类成分的化学分析[J]. 药物分析杂志,2004, 24(3): 229-237.ZHANG Ping , ZHANG Nanping, XIAO Xinyue, et al. Chemical Analysis of Saponins in Panax ginseng[J]. Chin J Pharm Anal, 2004, 24(3): 229-237(in Chinese).
    [4] 李树殿, 张永强, 鲁岐, 等. 中国、高丽、日本大力参化学成分的比较研究[J]. 人参研究, 1989, (8): 21-22.LI Shudian, ZHANG Yongqiang, LU Qi, et al. Study chemical composition of Dali ginseng in China, Korea and Japan[J]. Ginseng Research, 1989, (8): 21-22(in Chinese).
    [5] CHU C, XU S J, LI X N, et al. Profiling the ginsenosides of three ginseng products by LC-Q-TOF/MS[J]. J Food Sci, 2013, 78(5): 653-659.
    [6] 李丽, 刘春明, 吴巍, 等. 高效液相色谱电喷雾质谱联用法测定人参和西洋参的皂苷类成分[J]. 分析化学, 2005, 33(8): 1087-1090.LI Li, LIU Chunming, WU Wei, et al. Analysis of saponins in extract of Panax ginseng and Panax quinquofolium by liquid chromatography-electrospray ionization mass spectrometry[J]. Chinese J Anal Chem, 2005, 33(8): 1087-1090(in Chinese).
    [7] 张语迟, 刘志强, 宋凤瑞, 等. 利用高效液相色谱和电喷雾质谱指纹图谱鉴别人参与桔梗[J]. 质谱学报, 2008,29(4): 203-208.ZHANG yuchi, LIU Zhiqiang, SONG Fengrui, et al. Identification of Panax ginseng and Platycodon grandiflorum by the fingerprint of HPLC and ESI-MS [J]. Journal of Chinese Mass Spectrometry Society, 2008,29(4): 203-208 (in Chinese).
    [8] 吴巍, 王道武, 刘志强, 等. 人参皂苷的电喷雾负离子多级串联质谱研究[J]. 质谱学报,2003,24(增刊): 43-44.WU Wei, WANG Daowu, LIU Zhiqiang, et al. Studies on saponins using negative electropray ionization multi-stage tandem mass spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 2003, 24(Suppl): 43-44 (in Chinese).
    [9] 李丽, 刘志强, 许庆轩, 等. 人参中皂苷类化合物的HPLC-ESI MS研究[J]. 分析测试学报,2006,26(1):45-48.LI Li, LIU Zhiqiang, XU Qingxuan, et al. Determination of ginsenosides in extract of Panax ginseng by liquid chromatography-electrospray ionization mass spectrometry[J].Journal of Instrumental Analysis, 2006,26(1):45-48(in Chinese).
    [10] 张海江, 蔡小军, 程翼宇. 高效液相色谱-电喷雾质谱法鉴别人参、西洋参和三七的皂苷提取物[J]. 中国药学杂志, 2006, 41(5): 391-394.ZHANG Haijiang, CAI Xiaojun, CHENG Yiyu. Differentiation and authentication of saponin extracts from Panax ginsengPanax quinquefolius and Panax notoginseng by HPLC/ESI-MSn[J].Chinese Pharmaceutical Journal, 2006, 41(5): 391-394(in Chinese).
    [11] 郭继芬, 钟大放, 乔善义, 等.液相色谱-电喷雾质谱联用技术分析人参皂苷[J]. 质谱学报, 2003,24(4): 477-481.GUO Jifen, ZHONG Dafang, QIAO Shanyi, et al. Analysis of ginsenosides by liquid chromatography-electrospray mass spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 2003,24(4): 477-481(in Chinese).
    [12] WANG Y, PAN J Y, XIAO X Y, et al. Simultaneous determination of ginsenosides in Panax ginseng with different growth ages using high-performance liquid chromatography-mass spectrometry[J]. Phytochem Anal, 2006, 17(6): 424-430.
    [13] CUI M, SONG F R, ZHOU Y, et al. Rapid identification of saponins in plant extracts by electrospray ionization multi-stage tandem mass spectrometry and liquid chromatography/tandem mass spectrometry[J]. Rapid Commun Mass Spectrom , 2000, 14(14): 1280-1286.
    [14] 徐智秀, 肖红斌, 王加宁, 等. 高效液相色谱-质谱-质谱法分析人参皂苷[J]. 色谱,2000, 18(6): 521-524.XU Zhixiu, XIAO Hongbin, WANG Jianing, et al. Analysis of ginsenosides by high perfomance liquid chromatography/mass spectrometry/mass spectrometry(LC/MS/MS)[J].Chinese Journal of Chromatography, 2000, 18(6): 521-524(in Chinese).
    [15] 孙靖辉, 米钧, 秦秋杰, 等. RRLC-Q-TOF MS-法鉴别分析人参皂苷Rd和Re及其丙酰化产物[J].质谱学报, 2011, 32(2): 112-113.SUN Jinghui, MI Jun, QIN Qiujie, et al. Differentiation and identification of ginsenosides Rd, Re and related malonyl-ginsenosides by RRLC-Q-TOF-MS[J]. Journal of Chinese Mass Spectrometry Society, 2011, 32(2): 112-113(in Chinese).
    [16] 郑重, 宋凤瑞, 刘淑莹, 等. 人参、红参皂苷类成分指纹图谱研究[J]. 质谱学报, 2012, 33(6): 327-333.ZHENG Zhong, SONG Fengrui, LIU Shuying, et al. Fingerprint study on ginsenoside in ginseng and red ginseng[J]. Journal of Chinese Mass Spectrometry Society, 2012, 33(6): 327-333(in Chinese).
    [17] 刘丹, 濮社班, 钱士辉, 等. 中国红参化学成分的研究[J]. 中国中药杂志, 2011, 36(4): 462-464.LIU Dan, PU Sheban, QIAN Shihui, et al. Chemical constituents of Chinese red ginseng[J].China Journal of Chinese Materia Medica, 2011, 36(4): 462-464(in Chinese).
    [18] XIE Y Y, LUO D, CHENG Y J, et al. Steaming-induced chemical transformations and holistic quality assessment of red ginseng derived from Panax ginseng by means of HPLC-ESI-MS/MSn based multicomponent quantification fingerprint[J]. J Agr Food Chem, 2012, 60(33): 8213-8224.
计量
  • 文章访问数:  965
  • HTML全文浏览量:  4
  • PDF下载量:  1083
  • 被引次数: 0
出版历程
  • 刊出日期:  2014-07-19

目录

    /

    返回文章
    返回