基于UPLC/Q-TOF MS技术的白术药材化学成分快速识别研究

Rapid Identification of Components from Atractylodis macrocephalae Rhizoma Based on UPLC/Q-TOF MS

摘要: 建立了超高效液相色谱-串联四极杆飞行时间质谱(UPLC/Q-TOF MS)法快速鉴定白术生药材中的多种化学成分。采用Aquity UPLC/Q-TOF micro联用仪，以水-乙腈流动相梯度洗脱，ESI⁺模式采集数据，Masslynx4.1软件分析处理数据。实验分析了白术药材中的24个峰信号，鉴定了其中的20种化学成分。结果表明，应用UPLC/Q-TOF MS技术能够快速鉴定白术药材的化学成分，是中药成分分析的有效手段。

Abstract: The components of Atractylodis macrocephalae Rhizoma were identified by ultra-high performance liquid chromatography with tandem quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS). Using Aquity UPLC/Q-TOF micro system gradient elute with water-acetonitrile mobile phase under positive mode of ESI; data were analyzed by Masslynx4.1 software. Twenty-four peaks in chromatogram of Atractylodis macrocephalae Rhizoma were analyzed，and 20 kinds of components were identified. Rapid identification of components from Atractylodis macrocephalae Rhizoma was carried out by UPLC/Q-TOF MS, which will be an effective device in analysis of compositions research in traditional Chinese medicine.