矿物药青礞石对PTZ点燃癫痫大鼠脑组织中氨基酸神经递质含量的影响

刘圣金; 吴露婷; 吴德康; 王瑞; 喻斌; 王宇华; 刘培; 杨文国

doi:10.7538/zpxb.youxian.2016.0040

矿物药青礞石对PTZ点燃癫痫大鼠脑组织中氨基酸神经递质含量的影响

1.
南京中医药大学药学院,江苏省中药资源产业化过程协同创新中心,江苏南京210023
2.
南京中医药大学信息技术学院,江苏南京210023

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- 刊出日期: 2016-11-19

Effect of Mineral Chinese Medicine Chloriti Lapis on Amino Acid Content in Brain Tissues of Pentylenetetrazol-Kindled Epileptic Rats

1.
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
2.
Information Technology College, Nanjing University of Chinese Medicine, Nanjing 210023, China

摘要

摘要: 建立了超高效液相色谱-三重四极杆-线性离子阱质谱（Qtrap-UPLC-MS/MS）法同时测定大鼠脑组织中4种氨基酸类神经递质，研究了矿物药青礞石原药材粉末、水煎液、药渣对戊四氮（Pentylenetetrazol，PTZ）点燃癫痫模型大鼠脑组织中氨基酸类神经递质含量的影响。在ESI⁺电离模式下，采用多反应监测扫描方式测定脑组织（皮层，海马）中谷氨酸（Glu）、甘氨酸（Gly）、天冬氨酸（Asp）及γ-氨基丁酸（GABA）的含量。结果表明：4种氨基酸的线性关系良好，精密度、准确度均符合生物样品的分析要求。大鼠给予青礞石不同样品后，脑组织皮层和海马中兴奋性神经递质Glu、Asp水平显著降低。与模型组相比，皮层中Gly占氨基酸总含量的百分比均增加，Asp/Gly值、Glu/Gly值均降低；海马中，各组Glu、Asp、Gly、GABA的浓度均降低。这说明，矿物药青礞石能有效降低PTZ点燃大鼠脑内兴奋性氨基酸递质含量，调节皮层和海马区Gly含量。
- 超高效液相色谱-三重四极杆-线性离子阱质谱（Qtrap-UPLC-MS/MS） /
- 矿物药 /
- 青礞石 /
- 癫痫 /
- 氨基酸递质 /
- 海马 /
- 皮层
Abstract: The method of Qtrap-ultra high performance liquid chromatography tandem mass spectrometry (Qtrap-UPLC-MS/MS) was established for simultaneous determination four amino acid neurotransmitters in rat’s brain tissue. The effects of powder, water decoction and dregs of Chloriti Lapis on amino acid content in PTZ-kindled epileptic rat’s brain tissue were studied. The contents of Glu, Gly, Asp and GABA in rat’s brain tissue (cortex and hippocampus) were detected by multiple reactions monitoring (MRM) scan mode in positive electrospray ionization (ESI⁺). The result shows that the four amino acids have good linear relationship, the RSDs of precision and accuracy conform to the requirements of analysis in biological samples. After rats were treated with different samples of Chloriti Lapis, excitatory neurotransmitter Glu, Asp in cerebral cortex and hippocampus decreased significantly. Compared with the model group, the content of Gly in cerebral cortex is increased in the percentage of total amino acidcontents, while the ratio of Asp/Gly and Glu/Gly are decreased. The contents of Glu, Asp, Gly and GABA in other groups display reduction in hippocampus compared with the model group. This shows that mineral Chinese medicine Chloriti Lapis can effectively reduce the excitatory amino acid contents in PTZ-kindled rat’s brain, and effectively regulate the content of Gly in the cortex and hippocampus.
- Qtrap-ultra high performance liquid chromatography tandem mass spectrometry (Qtrap-UPLC-MS/MS) /
- mineral Chinese medicine /
- Chloriti Lapis /
- epilepsy /
- amino acid neurotransmitters /
- hippocampus /
- cortex

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矿物药青礞石对PTZ点燃癫痫大鼠脑组织中氨基酸神经递质含量的影响

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Effect of Mineral Chinese Medicine Chloriti Lapis on Amino Acid Content in Brain Tissues of Pentylenetetrazol-Kindled Epileptic Rats

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