Abstract: There are increasing evidences showing that the metabolism of trace elements in diabetic patients has being varied. These elements might play some specific roles in the pathogenesis of diabetes and its development. The differences of human serum trace element concentration between age range of 50-60 years old male with type 2 diabetes (95 cases) and healthy control group (91 cases) were studied. Concentrations of 19 trace elements in the serum samples were determined with acid assist microwave digestion by inductively coupled plasma mass spectrometry (ICP-MS) platform with a dilution factor of 25. The detection limit, precision and reproducibility were proved sufficient to serum trace element measurement. Compared with the control group, the patients of diabetes have higher concentration of elements Mn, Co, Cu, Ba, Pb and have lower concentration of elements V, Zn, As, Sb, W, there is no significant statistical differences between the diabetic patients and the healthy control group of elements Cr, Fe, Ni, Se, Mo, Cd, Sn, Hg, Tl. The results show that there are 10 trace elements which closely related to the diabetes mellitus have significant statistical concentration differences between the diabetic patients and the healthy control group with the age range of 50-60 years old male. This conclusion is helpful to study further on the pathogenesis of diabetes-related diseases which related on excess or deficiency of trace elements in the human serum, and also offer data support to further elaborate mechanisms and the development of diabetes which related with trace elements in the human serum.