Abstract: Relying on its high sensitivity and strong anti-jamming capability, gas chromatography-tandem mass spectrometry(GC-MS/MS) was widely used in chemical, biological and environmental analysis. This paper overviewed recent progress on the application of GC-MS/MS since 2000. Most of the relevant works were in the area of pesticide residues, environmental pollutants (including PAHs, PCBs, PBDEs, PCDD/Fs) and steroid hormones. The discussion mainly contained two aspects. First, the advantages of GC-MS/MS in the analysis of different targets were compared to other instruments. Second, the methods of sample treatment were widely used and suitable for GC-MS/MS analysis. Furthermore, domestic application of GC-MS/MS was stated simply，and the future development of application in GC-MS/MS was reviewed.