Abstract: The method for screening of 26 kinds of additives in wine was developed by liquid chromatography-quadrupole/orbitrap high resolution mass spectrometry. The additives were separated by C18 high performance column after the wines were diluted by methanol-water mixtures (1∶9, V/V). This method was quantitatived by external standard method using full scan mode of high resolution mass spectrometer extracting accurate mass extraction of the target compound. Good linearity is obtained in the range of 0.1-5 mg/L for 26 kinds of additives with the linear correlation coefficients greater than 0.99. Satisfactory recoveries range from 78.5% to 118.8% at three levels of 2, 4 and 20 mg/kg are gained in dry red and white wines with the relative standard deviation less than 12.7%. The limit of quantitation (LOQ) is 2 mg/kg. This method is suitable for screening of 26 kinds of additives in wines by its fast and simple preprocessing with high accuracy and precision of high resolution mass spectrometry.